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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
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- 保质期：
>1年
- 英文名：
Pullulanase/Limit-Dextrinase Assay Kit
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- 规格：
100/200 assays per kit
极限糊精酶/普鲁兰酶检测试剂盒介绍：
| Content: | 100 / 200 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Pullulanase/Limit-Dextrinase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase) |
| Application examples: | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. |
| Method recognition: | Novel method |
- High sensitivity
- Suitable for manual and auto-analyser formats
- No transglycosylation interference
- Very cost effective
- All reagents stable for > 1 year after preparation
- Very specific
- Simple format
- Standard included
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Explore more of our assay kit products for enzyme activity measurement.
极限糊精酶/普鲁兰酶检测试剂盒检测试剂盒说明书(PDF)请向客服索取！
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文献和实验1.A quick and simple gel diffusion assay to visualize and quantify pullulanase activity for resistant starch content in food crops.
Krishnan, V., Awana, M., Kulshreshta, A., Praveen, S. & Singh, A. (2022). Journal of Plant Biochemistry and Biotechnology, 1-7.
2.Effects of post-heading high temperature on some quality traits of malt barley.
Ni, S. J., Zhao, H. F. & Zhang, G. P. (2020). Journal of Integrative Agriculture, 19(11), 2674-2679.
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