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文献和实验CO-FISH, COD-FISH, ReD-FISH, SKY-FISH
Fluorescence in situ hybridization (FISH) has become a powerful tool for exploring genomes at the level of chromosomes. The procedure can be used to identify individual chromosomes, rearrangements between chromosomes, and the location
Materials 20 ng BAC DNA clone grown o.n. in 5 mL LB + chloramphenicol Autogen reagents Slide warmer Organics: MeOH, EtOH, formamide Fixative (3:1 MeOH:glacial HOAc) Biotin (or digoxigenin) nick-translation Kit (Invitrogen) 20x SSC
Parental Origin Determination FISH: Pod-FISH
of homologous chromosomes, the so-called parental origin determination fluorescence in situ hybridization (pod-FISH) technique. To perform this technique, all human chromosomes are covered with CNV-spanning BAC probes in one- to five-color chromosome-specific
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