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| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| B57 | 5 x 1 ml | B57 |
为确保酶切性能的稳定,Ferments公司的限制酶缓冲液含有BSA。BSA可增强限制酶的稳定性和结合DNA分离物中的杂质。缓冲液的多次冻-融不会导致BSA的沉淀。
Fermentas限制酶在推荐缓冲液中的酶活性达100%。
- 10 mM Tris-HCl (pH 8.0, 37°C)
- 5 mM MgCl2
- 100 mM KCl
- 0.02 % Triton X-100
- 0.1 mg/ml BSA
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文献和实验EMSA using ds Oligonucleotides【Harvard University】
Cepko/Tabin Lab ,Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html Cepko/Tabin Lab ,Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html Solutions 10X Annealing Buffer 200 mM Tris 8.0 200 ml 1M
EMSA using ds Oligonucleotides
complementary oligonucleotides with compatible half sites on the ends (I use BamHI and BglII). Dry down 300 ng of the two purified oligos together and resuspend in 9 m l Q with 1 m l 10X Annealing buffer. Place in a 65℃ water bath for 2 min and cool in 50 ml
EMSA using ds Oligo nucleotides
on the ends (I use BamHI and BglII). Dry down 300 ng of the two purified oligos together and resuspend in 9 m l Q with 1 m l 10X Annealing buffer. Place in a 65℃ water bath for 2 min and cool in 50 ml of the 65℃ water in a beaker on ice. This takes 15-20
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