- 询价
- Thermo scientific -fermentas
- SM0671
- 2025年10月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
|
| Catalog# | Size, concentration | Certificate of Analysis | MSDS |
| SM0671 | 50-100 applic. | SM0671 | |
| SM0672 | 250-500 applic. | SM0672 |
- Features
-
- 锐利条带，颜色稳定。
- 两条参考条带 – ~70 kDa的橙色条带和~10 kDa绿色条带。
- 即用型上样 – 无需煮沸。
- 在凝聚中易于示踪。
- Western转移效果好。
- Applications
-
- 监控蛋白的SDS-聚丙烯酰胺凝胶电泳迁移。
- 监控Western杂交的蛋白转膜效率。
- SDS-聚丙烯酰胺凝胶电泳和Western杂交确定蛋白大小。
PageRuler™ Prestained Protein Ladder是一 个三色分子量标准，含有10种蛋白，分子量范围为10 - 170 kDa。该分子量标准含有两条参考条带，分别为70 kDa的橙色参考条带和10 kDa的绿色参考条带。
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.
* Migration patterns were determined using respective NuPAGE® precast gels.
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文献和实验(2 μl) RNA ladder with 5 μg anchored Oligo(dT)20 primer at 70°C for 5 minutes. Keep on ice. 2) Combine the RNA-oligo dT complex from Step 1 with 1X 1st strand cDNA synthesis buffer supplemented with 10 mM DTT, 500 μM dNTP, 2 U RNaseOUT™
Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
into the wells of the SDS-PAGE gel. Load an appropriate amount of Trident Blue Prestained Protein Ladder or Trident Prestained Protein Ladder to one or more additional lanes.2. Run the gel in 1X Trident Running Buffer for 1-2 hours at 50-100 V.We recommend
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