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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
-PCR primer choice with respect to minimizing the problems associated with DNA contamination is to design primers that span at least one intron of the genomic sequence. This will result in a PCR product from genomic contamination that will be larger in size
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
sec. break x 3 times, 20% amplitude with a small tip). Now the length of your chromatin DNA should be 200-1000 bp. (13) Centrifuge for 20 min at 14000 rpm, 4°C. Recover supernatant. This is the soluble sheared chromatin fraction. This can be stored
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