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Kapa biosystems
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
X) 0.1 µL of Adapter oligo mix 2.5 µL of T4 DNA ligase (400 U/µL) Incubate at 20°C-23°C for 30 min and then at 16°C overnight. 43. Purify DNA using MinElute PCR Purification Kit
Multiplex Illumina Sequencing Using DNA Barcoding
cycler Gel electrophoresis apparatus (unit 2.5 ) Table 7.1.1 MaterialsOligonucleotide Sequences for Illumina Paired‐End Y‐Adapter and PCR Primers
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
DEPC‐treated H 2 O ( appendix 2D ) 10 µM GEX Adapter 1 (from Illumina Tag Profiling Sample Prep Kit, cat. no. FC‐102‐1005
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