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Kapa biosystems
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48 rxn
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文献和实验to be in the cross-linking step. MATERIALS Reagents Antibody ChIP cell lysis buffer ChIP dilution buffer ChIP hybridization buffer ChIP wash buffer Chloroform/isoamyl alcohol (24:1; prepare fresh before use
vol 687 Chapter 2 采用DNA聚合酶融合技术进行长片段PCR扩增 (精华)
. An 18.4 kb b-globin fragment was amplifiedin duplicate reactions from human genomic DNA (Promega G3041) using recommended reaction conditions and cyclingparameters (see Subheading 3). Adjustments in DNA template, primer, nucleotide, and DMSO
核蛋白基因组指纹技术Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
containing the linker adapter at a final concentration of 0.8 µM. Add 4 units of T4 DNA ligase (Roche) and incubate at 4°C for 24 hr. Use the ligated mixture directly as a template in a 100-ml PCR using 1 unit of Taq polymerase, 1x
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