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文献和实验Long-PCR Reagents and Guidelines
. Natl. Acad. Sci. 91, 5695-5699 (1994). 2. Crameri, A. and Stemmer, W. 10^20-Fold aptamer library amplification without gel purification. Nucleic Acids Research 21, 4410 (1993) 上一篇:反转录生产cDNA链以及PCR扩增 下一篇:Real time PCR-ABI Prism 7000
Real‐Time Quantitative PCR Analysis of Mitochondrial DNA Content
96‐well thin‐walled PCR plates Adhesive plate sealer ABI Prism 7900HT sequence detector
. The output of this step is a DNA amplicon library that reflects to a large extent the original stoichiometry of mature microRNAs in a cell or tissue (12 ). PCR amplification is performed with fluorescently labeled primers, which allows quantitative signal
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