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Kapa biosystems
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
. Amplify the DNA using Illumina PCR primers PE 1.0 and PE 2.0 and 2X Phusion HF Master Mix. i. Combine: 10.5 µL of DNA 12.5 µL of 2X Phusion HF Master Mix 1 µL
Multiplex Illumina Sequencing Using DNA Barcoding
sequences (depicted as blue lines) are then ligated onto both ends of DNA fragments. Purified ligation products are subjected to PCR amplification with primers (PCR1/2) containing the full sequences required for hybridization to the Illumina sequencing flow
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
) 10 mM dNTP mix (10 mM each dNTP; Invitrogen) GEX PCR Primers 1 and 2 (from Illumina Tag Profiling Sample Prep Kit, cat. no. FC‐102‐1005
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