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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BK1004
- 库存:
充足
- 供应商:
Kapa biosystems
- 规格:
2500 units
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文献和实验Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
. Meanwhile, preheat the PE Cetus Thermocycler 9600 to 95degC (Program #2). 2. Prepare the Taq polymerase dilution. AmpliTaq polymerase (N801-0060) is from Perkin-Elmer Cetus. 30ul AmpliTaq (5U/ul) 30ul 5X Taq reaction buffer 130ul ddH20
Cyanine Dye Purification Protocol
. 12 mL syringe (1 per dye) Pre-load protocol: Wash Sep-Pak with 10 mL of acetonitrile. Wash with 5 mL of water. Wash with 10 mL of 15 mM TEAA. Sample Preparation, Loading, and Elution: The column
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
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