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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
RT406
- 库存:
充足
- 供应商:
天根生化(科技)
- 规格:
60 U
产品简介:
本酶在以ATP作辅酶的情况下,可以催化相邻DNA链的5’磷酸基团和3’羟基之间的连接反应。平末端和粘性末端均可,此酶也可以催化双链RNA与双链DNA连接,但不能催化单链核酸的连接。
储存条件:
T4 DNA连接酶和10×T4 DNA ligase Buffer必须放置于-20℃保存,避免反复冻融,10×T4 DNA ligase Buffer建议分装使用。
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文献和实验Ligation of DNA with T4 DNA Ligase
recombination and DNA synthesis. DNA ligase from E. coli is a polypeptide with a molecular weight of 74,000 and is NAD-dependent. T4 DNA ligase is the product of gene 30 of the T4 phage, has a molecular weight of 68,000, and is ATP-dependent. Both enzymes
Joining RNA Molecules with T4 DNA Ligase
Researchers interested in studying RNA structure and function or RNA-protein interactions are increasingly using site-specifically modified RNAs to probe sites of interest (1 ). In addition to expanding the repertoire of functional groups
Splint Ligation of RNA with T4 DNA Ligase
Splint ligation of RNA, whereby specific RNA molecules are ligated together, can be carried out using T4 DNA ligase and a bridging DNA oligonucleotide complementary to the RNAs. This method takes advantage of the property of T4 DNA ligase
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