- ￥400
- Kapa biosystems
- 美国
- KK3604
- 2025年12月18日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
KK3604
- 库存：
充足
- 供应商：
Kapa biosystems
- 规格：
(100 x 25µL rxn)
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文献和实验to the Taq-buffer mix, and decrease the amount of H2O to 5.76 μl per sample. DO NOT use a hot start PCR protocol, or a hot start polymerase (e.g. AmpliTaq Gold), for the pre-amplification! The adapters are non-phosphorylated, so only the top strand is ligated
Complementation in Arabidopsis
ddH20 +Taq (ExTaq)* TOTAL 1µL 0.5µL 0.5µL 5µL 4µL 40µL 1µL 52µL *Add after 4 minutes of 95°C for Hot Start 3. Combine reactions
Genomic (Plant) DNA: Restriction Digestion for Sou
): At the conclusion of the digest either (a) Ethanol precipitate the DNA (which can also be stored at �20℃ to spin at a later date), (b) Load it on the gel (c) Freeze it away at �20oC to be processed later or (d) Add loading dye (if DNA is at the desired
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