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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
, cell-type-specific mapping of all the chromatin interactions mediated by specific proteins. The 6C assay advances our understanding of the three-dimensional aspects of the higher-order folding of chromatin and provides an important tool to examine
cDNA/AFLP:A tool for transcriptome analysis
for at least 30 seconds. 1.3.To the mixture add a base volume of chloroform (5 ml) and shake vigorously. To separate the phases, centrifuge the samples for 20 minutes (3500 rpm in a large swing-out rotor). Repeat chloroform extraction with one base
Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
8) Incubate the cells for 8 hours in a 5% CO2 incubator at 37ºC. 9) Transfer embryos to albumin agar plates and incubate for 48-72 hours more in a 37º C incubator at 37ºC. 10) Observe embryos using fluorescent microscope 48-72 hours
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