- ￥11440
- QIGEN
- 德国
- 9020070
- 2025年11月07日
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文献和实验Method: Preparation of Lymphoblastoid Cell Lines for Long Term Storage
in logarithmic phase of growth with a total volume of 80-100 ml/T-75 flask should yield enough cells to freeze 10 ampules (1.0 ml/ampule). Cells should have a count of 4 X 106 cells/ampule to 9 X 106 cells/ampule. Too high or too low a cell count lowers recovery
Protocols for ET recombination
the cells at 2.3 kV (Bio-Rad Gene Pulser), 25 uF with Pulse controller set to 200 ohms) Add 1 ml of LB medium and transfer back into the eppendorf tube. Incubate at 37℃ for 1 to 1.5 hours with shaking. Plate 100ul on suitable antibiot℃ plates, spin
Protocols for ET recombination
at 2.3 kV (Bio-Rad Gene Pulser), 25 uF with Pulse controller set to 200 ohms) （4）Add 1 ml of LB medium and transfer back into the eppendorf tube. （5）Incubate at 37oC for 1 to 1.5 hours with shaking. （6）Plate 100ul on suitable
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