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- QIGEN
- 德国
- 1061119
- 2025年12月31日
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文献和实验Use of Internal and External Standards or Reference RNAs for
and authenticating this control are critical to successful relative quantitation. In the paragraphs below, we describe commonly used internal controls and how these are authenticated. Internal Standard RNA Probes Inconsistencies in RNA isolation
content of a preparation can be determined on the basis of various different analytical procedures. Evaluation can be carried out via factor or via a calibration curve, with up to ten standards in the BioPhotometer. Absorption measurement at 280 nm
细胞/组织中的DNA定量（Quantification of DNA in cell / tissue samples）
Prepare the standards for the extended range assay from the stock solution to obtain a concentration of 4000 ng DNA in 40 μl volume for the first standard.Dilute the standards in DPBS in a row by two down to a concentration of 125 ng in 40 μl.Run standards
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