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文献和实验SSR GEL and Silver Staining Protocol
a small piece of vinyl tape on a lower outside corner of the acrylease treated glass gel plate to mark the untreated side and also help distinguish the plates. This helps avoid confusion between plates when using offset plates. The tape can remain in place
SSR GEL and Silver Staining Protocol
, deionized or distilled water, and ethanol, air dry. Use dedicated sponges for each treatment. 2. Using a kimwipe tissue, coat the inner side of notched or offset plate with acrylease (Stratagene) and allow to dry--I do not treat the top 2 inches
Method for Single-Cell Electroporation
at the tip. The micropipette is attached to a coarse manipulator with a pipette holder. A second silver wire is placed in direct electrical contact with the preparation. For SCE in tadpoles, the ground wire is placed near (approximately 1 cm) the tadpole
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