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- QIGEN
- 德国
- 239901
- 2025年11月12日
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文献和实验Clonality - X Chromosome Inactivation Assay
microdissected cells in 20 µl proteinase K buffer. Incubate overnight at 37°C. B: DNA Purification Lyse the cells overnight at 42°C, in 50 µl phenol:chloroform:isoamyl alcohol. Boil 10 min at 95°C. Vortex 1 min. Centrifuge 20 min at 14,000 rpm at RT
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
% SDS lysis buffer into 500 µl of supernatant (final 1% SDS). Mix gently. This step increases the SDS conc. in the sample to 1%, which is necessary to make the length of chromatin DNA short enough for ChIP (200 � 1000 bp) by sonication
in the bottom of the well. Materials DNA samples DNA size standard Loading dye solution Agarose TBE buffer Sybr Green I solution diluted 10,000-fold Dark Reader light box Plastic wrap
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