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文献和实验DNA Purification from Blood or Body Fluids
If the sample volume is less than 200 µl, add the appropriate volume of PBS. QIAamp Mini spin columns copurify RNA and DNA when both are present in the sample. RNA may inhibit some downstream enzymatic reactions, but not PCR. If RNA-free
Fetal DNA: Strategies for Optimal Recovery
of fetal DNA from maternal plasma and validated for this purpose in our laboratories. The use of the QIAamp DSP Virus kit (QIAGEN), the QIAamp DNA Blood Mini kit (QIAGEN), and the Magna Pure LC (Roche) is based on the kit components provided
of DNA from 6 ml of starting culture. Note : This is a typical mini-prep until step 7, where in step 7a you would precipitate the template and use it for Taq Cycle Sequencing with the Dye-Labeled Primers, or in step 7b proceed
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