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- QIGEN
- 德国
- 218540
- 2025年12月28日
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文献和实验DNA priming reaction x ul DNA 2 ul Reaction buffer, minus DTT 1 ul primer (20 ng) 10 ul total Heat 90-100℃ 2 min. Cool room temp. 30 min. Enzyme mix 4 ul radioactive nucleotide (12 l) 2 ul reaction buffer ( 6 l) 4 ul water
Primer Premier: Program for Design of Degenerate Primers from a Protein Sequence
oligonucleotide sequence for screening libraries and of a primer pair for amplification of target sequence by polymerase chain reaction (PCR). However, due to redundancy in the genetic code, oligonucleotide design must account for ambiguous DNA bases
into a 5 or 14 ml plastic tube with a pop-off cap (e.g. Falcon, #35-2063). 5. Immediately before performing the assay, transfer a small volume of the saturated starter culture into the tube containing agar. 100 ul of starter culture should be used
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