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文献和实验are used in the procedure. With the AllPrep DNA/RNA Micro Kit and AllPrep DNA/RNA Mini Kit, spin columns are used in the procedure. B AllPrep 96 DNA plate (left) and RNeasy 96 plate (right). By allowing the analysis of DNA and RNA
Isolation of Total RNA from Animal Cells use RNeasy Mini Kit
.) to 70 µl Buffer RDD. Mix gently. 8. Add the DNase I incubation mix (80 µl) directly onto the RNeasy membrane, 20–30°C for 15 min. 9. Run 700µl RW1 through the mini column, 15 seconds at 12,000 rpm. 10.
Materials Qiagen RNEasy Midi Kit Dounce homogenizers (for tissues) Syringes and needles (for cultured cells) 70% EtOH Sterile 15 mL conical tubes Procedure 1) Before using the reagents
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