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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名:
NSF (D31C7) XP®️ Rabbit mAb
- 抗原:
synthetic peptide corresponding to residues surrounding Leu524 of human NSF protein
- 应用范围:
W, IP, IF-F
- 保质期:
详见说明书
- 库存:
大量
- 级别:
详见MSDS文件
- 适应物种:
H,M,R,Mk
- 供应商:
CST
- 是否单克隆:
多克隆
- 保存条件:
-20°c
- 规格:
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格: | 产品价格: | 询价 | |
|---|---|---|---|
| 规格: | 40 ul (4 western blots) | 产品价格: | 询价 |
| 规格: | 100 ul (10 western blots) | 产品价格: | 询价 |
| 规格: | carrier free & custom formulation / quantity | 产品价格: | 询价 |
pathwaymore infoapplication referencesdatasheet PDFMSDS PDFprotocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | H M R Mk | Endogenous | 78 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | NSF (D31C7) XP® Rabbit mAb detects endogenous levels of total NSF protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu524 of human NSF protein. |
| Background | Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the α-SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the α-SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the α-SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h)即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复:简单易行效果好,CST 推荐使用微波炉完成修复。合适的修复液:根据抗体说明书使用合适的修复液。用柠檬酸修复后,切片需浸泡在修复液中,自然冷却;而用 EDTA 修复后,切片可直接从修复缸中取出,直接进行下一步。注:使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb(#4267),EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
的染色强度。获得合适的染色强度后,将载玻片置于蒸馏水 (dH2O) 中以阻止进一步显色。抗体:Phospho–Stat3(Tyr705)(D3A7) XP® Rabbit mAb #9145样本:石蜡包埋的人类乳腺肿瘤显色检测:利用 SignalStain® DAB Substrate Kit #8059(左),竞争者提供的 DAB(中和右)CST 的 IHC/IF 明星产品推荐:生化试剂:Catalog NumberProduct NameProduct
实际上将作为阴性对照使用。CST 推荐使用 Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620。该抗体可检测与基因组中所有 DNA 序列结合的所有组蛋白 H3 类型(H3、H3.3、CENP-A)。因此,不管检测的位点活性状态如何,该抗体都能为 ChIP 实验提供通用的阳性对照。 阴性对照 阴性对照抗体(如正常兔 IgG)不能识别特异抗原表位,因此可用于检测非特异性结合。例如,如果阴性对照样品中的产物量等于特异
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