- ￥5000
- GeneTex
- 美国
- GTX60339
- 2025年07月15日
- WB, ELISA, ChIP assay
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The AML1-ETO fusion protein using a KLH-conjugated synthetic peptide.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60339
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ELISA, ChIP assay
- 靶点：
This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)).
- 抗体英文名：
AML1-ETO antibody - ChIP grade
- 抗体名：
AML1-ETO 抗体 - ChIP grade
- 规格：
50 μl
ChIP analysis of sheared chromatin from 1.25x10⁶ Kasumi-1 cells using GTX60339 AML1-ETO antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. This figure shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.
Antibody amount : 4μl
WB analysis of nuclear extracts of SKNO-1 cells (15 μg) using GTX60339 AML1-ETO antibody - ChIP grade.
Dilution : 1:1,000
ELISA analysis of peptides used for immunization using GTX60339 AML1-ETO antibody - ChIP grade.
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文献和实验Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
Characterization and Quality Control of Antibodies Used in ChIP Assays
, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Abstract Table of Contents Materials Figures Literature Cited Abstract ChIP?chip combines
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