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鼎国昌盛
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文献和实验固定10分钟; 2.蒸馏水洗; 3.60%异丙醇浸洗; 4.1由红O染液10分钟(染液可回收再利用); 5. 60%异丙醇分色至背景五色; 6.蒸馏水洗; 7.Mayer苏木精复染; 8.自来水洗(蓝化)1~3分钟; 9.蒸馏水洗; 10.甘油明胶封片。 结果:组织细胞中脂滴呈橘红色,核呈蓝色。 油红O脂类染色澄 示大鼠脂肪肝中肝细胞内染为红色大脂
Recombineering/Lambda red-mediated gene replacement
Overview Single-gene knockouts using λ red system, adapted from Datsenko and Wanner paper. The goal of this protocol was to create an endA (endonuclease I) knockout, but obviously it can be adapted to any gene. The knocked-out gene
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells
banks of hESC lines with matched or reduced incompatibility could potentially reduce or eliminate the need for immunosuppressive drugs and/or immunomodulatory protocols altogether, for example, O-type RhD− lines for generation of universal red blood
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