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- 国产
- DS023
- 北京
- 2025年08月07日
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- 供应商：
鼎国昌盛
- 库存：
大量
- 规格：
500ml
DS023 Ethanol l Anhydrous 无水乙醇 500 18.00
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文献和实验【小常识】Ethanol Precipitation of DNA
Ethanol Precipitation of DNA Ethanol precipitation is frequently used for concentration of DNA solutions and for removal of protein, salt, and unincorporated nucleotides. The two most common protocols use either 0.3 M sodium acetate
Ethanol precipitation method for purifying PCR products
1. For each PCR reaction (50 µL) prepare a 1.5mL microcentrifuge tube containing the following: - 5 µL of 3M sodium acetate (NaOAc), pH 4.6 - 100 µL of 95% ethanol (EtOH) 2. Pipet the entire contents of each PCR reaction
should have no more than 125 µL of DNA solution, for example). 2.Add one tenth volume of Sodium Acetate buffer to equalize ion concentrations. 3.Add at least two volumes of cold 100% ethanol; let stand in -20℃ freezer for at least one hour. 4.Centrifuge sample for 15
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