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- prospec
- LPB65-100
- 以色列
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Low protein binding microtubes for in gel digestion
- 库存：
大量
- 供应商：
北京鼎国昌盛
- 规格：
100
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文献和实验In-gel digestion of proteins for peptide fingerprint mapping
(see eg ., Shevchenko et al. 1996a and b). In the protocol below, we describe the in-gel digestion procedure, as it is routinely performed in our laboratory, for peptide mass mapping of picomole to subpicomole quantities of protein derived from coomassie- or silver
over the pH gradient. Regions with low buffer capacity are more prone to distortion. In preparative experiments with protein loads, buffering capacity form the proteins may affect the pH gradient. Local heating will occur in the regions with the highest
with low buffer capacity are more prone to distortion. In preparative experiments with protein loads, buffering capacity form the proteins may affect the pH gradient. Local heating will occur in the regions with the highest field strength (lowest
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