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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
47 cm vinyl, 0.4 mm thick, 1 set
- 规格:
2
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文献和实验and allowing more readable sequence information to be obtai ned from one gel. DNA sequencing gels are cast between the 38 x 50cm and 38 x 47.5cm glass plates of the Bio-Rad SequiGen?sequencing system. You will need: A standard detergent 2% dichlorodimethyl
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
in 9cm petri w/ 20ml aleurone solution: 10mM CaCl2 (1.47g/l) 1X antibiotic solution 100X antibiotic stock: 5mg/ml ampicillin 10mg/ml neomycin 1mg/ml nystatin GA 7mg/1ml EtOH, warm at 37C to dissolve, use 20ul/20ml aleurone
overnight. Do not use the 40 volt protocol with the BioRad unit.12. UV fix the wet membrane (leave it on the wet 3MM support) with 120,000 oules UV via the UV crosslinker. Alternatively, use a Sylvania germicidal UV lamp exactly 12 cm
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