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- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
0.2 ml 12-Strip Tubes with Domed Caps
- 供应商：
北京鼎国昌盛
- 规格：
960
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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
top centrifuge.2.Remove the supernatant by decanting or pipetting. Add 5 ml RNase-free water to the pellet, and close the tube using a fresh secondary Hemogard closure.Gently decant the supernatant, and blot on a paper towel.3.Thoroughly resuspend the pellet
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
1、A smear (rather than a single colony) of BAC colonies are picked and transferred into a 12X75 mm Falcon tube containing 3 ml of LB medium (10 g Bacto-Tryptone, 5 g Bacto-yeast extract, and 10 g NaCl in 1 L H2O, autoclaved) with appropriate
of the apparatus used. Figure F.2 presents a Nomogram for calculation of R.C.F. for a given radius and RPM. A simple formula for calculating this value is: RCF = 1.12r (RPM/1000)where r = radius in millimetersRPM = revolutions per minute The difficulty
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