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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Electroporation cuvettes, 4mm, sterile
- 供应商：
北京鼎国昌盛
- 规格：
50
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文献和实验Streptomyces:Protocols/Transformation by Electroporation
. Safety General laboratory & molecular microbiology safety rules apply. Requirements LB/LB-NaCl, LBA/LBA-NaCl, 10% glycerol (sterile), 15mL falcon tube(s), electrophoration cuvette (for each transformation), micro-centrifuge tube(s) (MCT
Transformation of E. coli by Electroporation
for electroporation. 1. Use a fresh colony of DH5α (or other appropriate host strain) to inoculate 5 ml of SOB (without magnesium) medium in a 50 ml sterile conical tube. Grow cells with vigorous aeration overnight at 37EC. 2. Dilute 2.5 ml of cells into 250
Mesoplasma florum:Electroporation
level. Select cultures which are just changing color. chilled mycoplasma electroporation buffer 8 mM HEPES pH 7.4 272 mM sucrose chilled 1 mm electroporation cuvettes 10 ml chilled ATCC 1161 medium
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