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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
北京鼎国昌盛
- 库存:
大量
- 英文名:
Dual Reference comb, 1.5 mm thick
- 规格:
1
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文献和实验You will need the following reagents: 5x Sample Buffer 10% w/v SDS 10 mM Dithiothreitol, or beta-mercapto-ethanol
DOP-PCR OF DNA FOR COMPARATIVE GENOMIC HYBRIDIZATION
In designing the PCR reaction, be sure to include a negative PCR control (blank without DNA), a positive control (we use MPE-600 breast tumor cell line DNA) and enough reference DNA for all of the planned CGH reactions. METHOD
BAC DNA Purification For Injection
in the morning--prolonged time at saturation will make it difficult to isolate BAC DNA. Spin 15 min at 5500xg to pellet cells; decant supernatant Resuspend cell pellet in 50 ml P1 Qiagen buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA) Add 50 ml P2
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