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- prospec
- CBLSR-150
- 以色列
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
北京鼎国昌盛
- 库存：
大量
- 英文名：
Single Reference comb, 1.5 mm thick
- 规格：
1
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文献和实验Units 1 mg = 10-3 g 1 ug = 10-6 g 1 ng = 10-9 g 1 pg = 10-12 g 1 kb of double stranded DNA = 660 kD 1 kb of single stranded DNA = 330 kD 1 kb of single stranded RNA = 340 kD Average MW
Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
as follows:MDE gel 16.0mlddH2O 44.2ml10X TBE 3.84ml10% APS 256µlTEMED 25.6µlPour sequencing gel format with appropriate sharkstooth comb. Gel will polymerize in about 1 hour.Loading Buffer95% formamide10mM NaOH0.025% Bromophenol Blue0.025% Xylene CyanolRun
Agarose Gels for Single Stranded DNA
Water 1.00 ml 10 mg/mL Ethidium Bromide 3. Melt agarose in the microwave 4. Seal horizontal gel apparatus. Pour molten agarose onto gel plate to a depth of 4 - 8 mm. 5. Insert a comb until its base is 1 mm from the base of the gel. Allow
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