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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
ABI Casting Comb
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 规格:
1
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文献和实验dryer (Life Technologies)Glass plates, 31.0 x 38.5 cm (Life Technologies)0.4 mm spacers (Life Technologies)Casting boot (Life Technologies)Shark tooth comb (Life Technologies)Small clampsWhatman blotting paper, 3 mm thicknessKodak Biomax MR or AR
, and ethanol to remove potential fluorescent contaminants prior to taping. Denaturing 6% polyacrylamide gels are poured into 0.3 mm X 89 cm X 52 cm taped plates and fitted with 36 well forming combs. After polymerization, the tape and the comb are removed
Agarose gel electrophoresis(琼脂糖凝胶电泳)
from the gel. Prior to gel casting, dried agarose is dissolved in buffer by heating and the warm gel solution then is poured into a mold (made by wrapping clear tape around and extending above the edges of an 18 cm X 18 cm glass plate), which is fitted
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