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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
北京鼎国昌盛
- 英文名:
Goldmine 10X CE running buffer w/EDTA
- 库存:
大量
- 规格:
1
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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
8.3 with acetic acid at room temp.Adjust volume to 500 ml.Running buffer is 1X TGOE buffer[Note that there is no Mg or EDTA in both the gel and in the running buffer.]
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
% ammonium persulfate 30 microliters TEMED pH 8.3 with acetic acid at room temp. Adjust volume to 500 ml. Running buffer is 1X TGOE buffer [Note that there is no Mg or EDTA in both the gel and in the running buffer.]
-Cl, pH8.0 1.2114 20% (v/v) Glycerol 20g 4% (w/v) SDS 4g 0.2% Bromophenol blue 0.2g 200mM DTT 20ul 5x Running buffer: 1 L 15.1 g Trizma base (= 125m M) 94g Glycine (= 1.25 M) 50ml 10%SDS (= 0.5%)--add last Do not adjust the pH!! 10x Blotting
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