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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
北京鼎国昌盛
- 英文名:
CleanSpin, 200 preps
- 规格:
1
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文献和实验,000 rpm, 10 min. 7) Transfer supernatant to new tube. Extract with 500 µl PCA (Phenol:Chloroform:Iso-amyl alcohol, 50:49:1). Spin 14,000 rpm, 5 min. Note: The PCA step is optional if the preps are used for restriction digests
Note: The PCA step is optional if the preps are used for restriction digests - however, the plasmid DNA is not likely to be stable over time without PCA extraction due to potential co-purifying DNases. 8) Transfer 800 µl aqueous
A. DNA PREPARATION PROTOCOL: The squishing buffer (SB) is 10 mM Tris-Cl pH 8.2, 1 mM EDTA, 25 mM NaCl, and 200 ug/ml Proteinase K, with the enzyme diluted fresh from a frozen stock each day. Place one fly in a 0.5 ml tube
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