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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
CleanSpin, 50 preps
- 供应商:
北京鼎国昌盛
- 规格:
1
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文献和实验Ograve;alkali-lysis%26Oacute; to release DNA which is then separated from the rest of the cellular components. Traditional methods have included the use of organic solvent extractions (e.g. phenol extraction was used for mini-preps) or ultracentrifugation (e.g
,000 rpm, 10 min. 7) Transfer supernatant to new tube. Extract with 500 µl PCA (Phenol:Chloroform:Iso-amyl alcohol, 50:49:1). Spin 14,000 rpm, 5 min. Note: The PCA step is optional if the preps are used for restriction digests
). Incubate on ice, 5 min or more. 6) Spin 14,000 rpm, 10 min. 7) Transfer supernatant to new tube. Extract with 500 µl PCA (Phenol:Chloroform:Iso-amyl alcohol, 50:49:1). Spin 14,000 rpm, 5 min.
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