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- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京鼎国昌盛
- 英文名：
SDS Buffer Neutral
- 规格：
1
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文献和实验Southern Blot using Neutral Transfer
is better for resolving larger fragments. Add ethidium to buffer near red ( ). (0.1 μg/ml ethidium should be included in the gel for visualization). 3) Run gel 5-6 hours at 40 to 60 volts. Once gel is finished running, be sure to take a picture with ruler
SDS Gel Electrophoresis of Tubulin\MAPs
Buffer 0.025 M Tris 0.192 M Glycine 0.1% SDS Adjust pH to 8.3 0.2-0.3 ml samples of brain extract, and containing 200 micrograms protein 0.0625 M Tris-HCl (pH 6.8)
SDS-PAGE Western Blotting Protocols
cells or fruiting bodies. Klett 100 cells are at a density of 5E8. 2. Pellet at RT for 1-2’ and remove s/n. 3. Extract protein by SDS/Urea Method, TCA-precipitation, or sonication. 4. Measure [protein] by Bradford (use 1-2 ul of each sample
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