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- 2026年08月28日
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Dual Media Set(100ml EB&500ml OB)
• Simple procedure: inoculate bacteria into EB™ medium for cell expansion, add OB™ medium for protein over-expression
• Eliminates the need to monitor culture density and to optimize timing of inducer addition
• Synchronizes cultures expressing diverse recombinant proteins
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文献和实验Dual- and Triple-Color Fluorospot
stimulatory agents of choice and anti-CD28 (0.1 μg/ml; seeNote 4), in a final volume of 100–150 μl/well. The number of cells per well is dependent on the stimulating agent used (seeNote 5). b. Incubate the plate overnight at 37°C in a humidified
Cell Culture Media and Solutions
sterilize through a 0.22 � filter and store up to 2 weeks at 4 degrees C. Growth media: (1 liter) 165.0 ml fetal bovine serum, heat inactivated at 50-60 degrees C for one half hour 12.0 ml 200mM (100 X) L-glutamine 1.2 ml 50 mg/ml
Yeast Media, Solutions and Stocks
after autoclaving the media. Use the HCl salts of amino acids wherever applicable. Stock solution ml stock for Constituent Final mg/L per 100 ml dH
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