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- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA-specific B cell probe (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建:A DNA sequence encoding the Influenza A virus (A/Perth/16/2009 (H3N2)) hemagglutinin (ACS71642.1) (Met1-Lys519, with a mutation Tyr 108 Phe), was expressed with a polyhistidine tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 17
蛋白分子量:The recombinant hemagglutinin of Influenza A virus (A/Perth/16/2009 (H3N2)) consists of 514 amino acids and predicts a molecular mass of 58 kDa.
蛋白NP号:ACS71642.1
蛋白氨基酸序列:Met1-Lys519
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验方法在宿主细胞中表达,扩增得到大量相同的DNA片段,称为DNA克隆,亦称分子克隆。 基本原理与过程: 1、分离纯化目的基因 2、目的基因 vector =重组DNA分子 3、重组DNA分子导入受体细胞,并在其内增殖。 4、筛选含有重组DNA的细胞——细胞克隆(cell clone),将转化的细胞置于琼脂表面,以刺激细胞克隆生长,这些细胞是由单个细胞形成的遗传相同的细胞群体,故称细胞克隆。再将每个克隆移至液体培养基中进行扩增。 5、分离重组DNA克隆:即收获扩增的培养细胞,并选择分离
Site‐Specific Protein Labeling with SNAP‐Tags
cell imaging and for in vitro analysis, are described. This includes a description of a releasable SNAP?tag probe that allows the user to chemically cleave the fluorophore from the labeled SNAP?tag fusion. In vitro labeling of purified SNAP?tag fusions
active form of human recombinant PON-1 in the mammalian cell line CHO. METHODS Construction of the paraoxonase : cloning vector The coding region for human paraoxonase-1 was amplified from human liver cDNA using a set of gene specific
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