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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA-specific B cell probe (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建:A DNA sequence encoding the Influenza A virus (A/Perth/16/2009 (H3N2)) hemagglutinin (ACS71642.1) (Met1-Lys519, with a mutation Tyr 108 Phe), was expressed with a polyhistidine tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Gln 17
蛋白分子量:The recombinant hemagglutinin of Influenza A virus (A/Perth/16/2009 (H3N2)) consists of 514 amino acids and predicts a molecular mass of 58 kDa.
蛋白NP号:ACS71642.1
蛋白氨基酸序列:Met1-Lys519
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验方法在宿主细胞中表达,扩增得到大量相同的DNA片段,称为DNA克隆,亦称分子克隆。 基本原理与过程: 1、分离纯化目的基因 2、目的基因 vector =重组DNA分子 3、重组DNA分子导入受体细胞,并在其内增殖。 4、筛选含有重组DNA的细胞——细胞克隆(cell clone),将转化的细胞置于琼脂表面,以刺激细胞克隆生长,这些细胞是由单个细胞形成的遗传相同的细胞群体,故称细胞克隆。再将每个克隆移至液体培养基中进行扩增。 5、分离重组DNA克隆:即收获扩增的培养细胞,并选择分离
Site‐Specific Protein Labeling with SNAP‐Tags
cell imaging and for in vitro analysis, are described. This includes a description of a releasable SNAP?tag probe that allows the user to chemically cleave the fluorophore from the labeled SNAP?tag fusion. In vitro labeling of purified SNAP?tag fusions
) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
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