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- Sino Biological
- 50445-M20B
- 2025年08月12日
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- 规格：
50 µg
蛋白名称：ERK2蛋白, ERK2 protein
蛋白构建：A DNA sequence encoding the mouse MAPK1 (P63085) (Met1-Ser358) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：No Kinase Activity
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant mouse MAPK1/GST chimera consists of 595 amino acids and has a calculated molecular mass of 69.1 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：P63085
蛋白氨基酸序列：Met1-Ser358
蛋白标签：N-GST & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验MAP-kinase antibody and anti-Dictyostelium ERK2 antibody. In this chapter, we describe the methods we have used to assess the level of activated ERK2 and partial involvement of G protein in cAMP-induced ERK2 activation.
yirenzhe ERK1与ERK2到底有什么区别？除分子量不同外。 doctormy ERK1 和 ERK2 的区别是个很大的研究课题，还有很多未知啊，呵呵 目前研究认为，ERK1 和ERK2 的上游激活路径高度相似，而且他们目前已知的下游靶点也非常一致。但当前有关区别的研究主要还是侧重于具体的生物学功能方面：人们发现ERK2与人体发育密切相关，对于学习和记忆的功能非常重要，而ERK1则不然；有学者认为
Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
to be CRM1- and MEK1/2-dependent. Here, we describe a general nuclear import assay of wild-type ERK2 that can be employed to identify different mechanisms governing nuclear entry of the protein kinase, adapted to evaluate ERK2 mutants that impair nuclear
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