Recombinant Human PPARG / Nr1c3 / PPARgamma Protein (His & GST Tag) | 重组人 PPARG / Nr1c3 / PPARgamma 蛋白 (His & GST标签)
- ￥3870
- Sino Biological
- 北京
- 12019-H20B
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human PPARG / Nr1c3 / PPARgamma Protein (His & GST Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50 µg
蛋白名称：Human PPARG / Nr1c3 / PPARgamma Protein (His & GST Tag)
蛋白构建：A DNA sequence encoding the human PPARG isoform 2 (P37231-1) (Met 1-Tyr 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 65 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Met
蛋白分子量：The recombinant human PPARG/GST chimera consists of 742 amino acids and has a calculated molecular mass of 85.4 kDa. It migrates as an approximately 85.4 kDa band in SDS-PAGE under reducing conditions as predicted.
蛋白NP号：P37231-1
蛋白氨基酸序列：Met1-Tyr505
蛋白标签：N-GST & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Thrombin Cleavage of GST-Fusion protein
to cleave 1 mg of fusion protein at room temperature overnight. 1 X PBS buffer: 140mM NaCl; 2.7mM KCL; 10mM Na2HPO4; 1.8mM KH2PO4; pH 7.3 Thrombin Cleavage of Free Eluted Fusion protein First you have to calibrate amount of thrombin, temperature
High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (>10,000 constructs) and has resulted in the deposition of 112 unique structures
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
: Protein Interactions, Second Edition Edited by Erica A. Golemis and Peter D. Adams ABSTRACT Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible
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