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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Dengue virus (DENV) (type 2, strain New Guinea C/PUO-218 hybrid) E / Envelope Protein (Domain III, His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:DENV Envelope蛋白, DENV Envelope protein
蛋白构建:A DNA sequence encoding the envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid)(AAC59274.1) (Met578-Gln680) was expressed with a polyhistidine tag at the C-terminus.
表达宿主:Yeast
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:Please contact us for more information.
预测N端:Met 578
蛋白分子量:The recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid) consists of 114 amino acids and predicts a molecular mass of 13 kDa.
蛋白NP号:AAC59274.1
蛋白氨基酸序列:Met578-Gln680
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Whole-Genome Analysis to Identify Type III-Secreted Effectors
Many Gram-negative plant and animal pathogens share a common virulence strategy that relies on the specialized type III secretion system. This apparatus is used to secrete virulence factors, called effectors, into the extracellular host
His 标签蛋白纯化效果不理想,宝宝心里苦呀。今天,小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面: 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高,容易导致蛋白炭化;功率过低,蛋白释放不出来。 建议 改变超声功率,同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素,如:金属离子螯合剂、强还原剂、过高的咪唑浓度。同时,优化缓冲液的pH、盐
Tracking the Secretion of Fluorescently Labeled Type III Effectors from Single Bacteria in Real Time
A large number of Gram negative pathogens use a specialized needle-like molecular machine known as Type III Secretion (T3S) system. This highly sophisticated molecular device consists of a basal body spanning the two bacterial membranes
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