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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse SELP / selectin P / P-selectin Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/50.00 µg/100.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥25190.0 |
|---|---|---|---|
| 规格: | 50.00 µg | 产品价格: | ¥1400.0 |
| 规格: | 100.00 µg | 产品价格: | ¥3870.0 |
蛋白名称:P-Selectin蛋白, P-Selectin protein
蛋白构建:A DNA sequence encoding the mouse SELP (Q01102) (Met1-Ala709) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELP-coated plates (5 μg/mL and 100 μL/well), approximately >60% cells will adhere specifically after 60 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Trp 42
蛋白分子量:The recombinant mouse SELP comprises 679 amino acids and has a predicted molecular mass of 74 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:Q01102
蛋白氨基酸序列:Met1-Ala709
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Single Primer ("Semi-Random") P
to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL Cycle as follows: 30"x94°C 20 cycles 0"x94°C 0"x55°C 1'x72° S=9 30 cycles 0"x94°C 0"x40°C 1'x72° S=6 30 cycles 0"x94°C 0"x55°C 1'x72° S=9 Clean up: Add 1 µL ExoI
T1.1 =26.2 T1.2 =43-26.2=16.8 T2.1 =53-26.2=26.8 T2.2 =44-26.2=17.2 3.计算x2 值 按公式20.12代入 4.查x2 值表求P值 在查表之前应知本题自由度。按x2 检验的自由度v=(行数-1)(列数-1),则该题的自由度v=(2-1)(2-1)=1,查x2 界值表(附表20-1),找到x2 0.001
。( ) 7.x2值的取值范围是x2≥0。( ) 8.x2值和t值一样,随着n´增加而增加。( ) 9.在x2值表中,当n´一定时,x2值越大,P值越小。 ( ) 10.R×C表的x2 检验中,P<0.05,说明被比较的n个(n>3)样本率之间。至和某两个样本间差别有显著性。( ) 三、习题 1.为比较槟榔煎剂和阿的平驱绦虫的效果,对45名绦早患者进行治疗,其结果如下,问两药疗效是否相同
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