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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human RANKL / OPGL / TNFSF11 / CD254 Protein (mFc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Human RANKL / OPGL / TNFSF11 / CD254 Protein (mFc Tag)
蛋白构建:A DNA sequence encoding the human TNFSF11 (AAC51762.1) (Gly63-Asp244) was expressed with the Fc region of mouse IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:1.Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF11B His (Cat:10271-H08H) at 2 μg/ml (100 μl/well) can bind Human RANKL mFc(Cat:11682-H04H), the EC50 of Human RANKL mFc is 15-80 ng/mL. 2. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells. The ED50 for this effect is typically 15-45 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Asp
蛋白分子量:The recombinant human TNFSF11/mFc comprises 418 amino acids and has a predicted molecular mass of 47.1 kDa. The apparent molecular mass of the monomer is approximately 50-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:AAC51762.1
蛋白氨基酸序列:Gly64-Asp245
蛋白标签:N-mouse IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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