- ￥5820
- Sino Biological
- 北京
- 40069-V08B1
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ 至 -80℃
- 保质期：
12个月
- 英文名：
MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg
蛋白名称：Coronavirus spike蛋白, Coronavirus spike protein
蛋白构建：A DNA sequence encoding the spike protein S1 Subunit MERS-CoV (AFS88936.1)(Met1-Glu725) was fused with a polyhistidine tag at the C-terminus.
表达宿主：Baculovirus-Insect Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: 40069-V08B1) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4 (Cat: 10688-HNCH). The EC50 of of biotinylated DPP4 (Cat: 10688-HNCH) is 0.52-1.22 μg/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725） (Cat: 40069-V08B1) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H). The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.02-0.06 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Tyr 18
蛋白分子量：The recombinant spike protein S1 Subunit MERS-CoV comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：AFS88936.1
蛋白氨基酸序列：Met1-Glu725
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Liv, L;Electrochemical immunosensor platform based on gold-clusters, cysteamine and glutaraldehyde modified electrode for diagnosing COVID-19. Microchemical journal : devoted to the application of microtechniques in all branches of science, PMID: 34054147
3, Liv, L; et al. Electrochemical biosensing platform based on hydrogen bonding for detection of the SARS-CoV-2 spike antibody. Analytical and bioanalytical chemistry, PMID: 34741650
4, Weskamm, LM; et al. Persistence of MERS-CoV-spike-specific B cells and antibodies after late third immunization with the MVA-MERS-S vaccine. Cell Reports Medicine, PMID: 35858586
5, Karunakaran, V; et al. A non-invasive ultrasensitive diagnostic approach for COVID-19 infection using salivary label-free SERS fingerprinting and artificial intelligence. Journal of photochemistry and photobiology. B, Biology, PMID: 36049288
6, Shim, JE; et al. Single-Nanoparticle-Based Digital SERS Sensing Platform for the Accurate Quantitative Detection of SARS-CoV-2. ACS applied materials & interfaces, PMID: 35951983
7, D'Arco, A; et al. Secondary Structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 Spike Proteins Revealed by Infrared Vibrational Spectroscopy. International journal of molecular sciences, PMID: 37298500
8, Tian, Y; et al. Comparison of N- and O-Glycosylation on Spike Glycoprotein 1 of SARS-CoV-1 and MERS-CoV. Journal of proteome research, PMID: 40193531
His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
乙型肝炎、和HBsAg阳性的患者血清中前S1蛋白，前S1抗原阴转愈早，AHB患者的疗程愈短，预后也愈好。说明前S1抗原及其抗体的检测是急性乙型肝炎的临床诊断，疗效观察和判数据预后的良好指标。 前S1蛋白的分子生物学特性 1．乙肝病毒（HBV）的基因结构 HBV为嗜肝DNA病毒，由一个不完全双链DNA组成，约3200个氨基酸。长链L含4个开放读码框架，为病毒蛋白的编码区：S区、C区、P区、X区。短链S相当于长链的50%-100%，其不固定端可被内原性DNA多聚酶延长，使病毒成为完整
模子里配制的5%聚丙烯酰胺凝胶-8 mol/l尿素体系以及微型蛋白凝胶装置中简易快速的执行。 即使使用单链探针，用S1核酸酶分析真核RNA结构还是会有假象。例如，DNA:RNA杂合双链中微小的不配对就会对S1核酸酶有抗性。相反地，富含rU:dA序列的完美配对的杂合双链区容易受到酶切。一条单链DNA分子如果同时和两条不同的RNA分子杂交，则可以不被核酸酶作用。最后一点，S1核酸酶不能有效地切割与RNA突出环相对的一段DNA。大多数这些问题可以用改变S1核酸酶浓度、采用不同酶切浓度、使用
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