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100 µg
蛋白名称:CLM-9蛋白, CLM-9 protein
蛋白构建:A DNA sequence encoding the human CD300LG (AAH25395.1)(Met1-Arg247) was expressed with six amino acids (LEVLFQ) at the C-terminus was expressed and purified.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 19
蛋白分子量:The recombinant human CD300LG consists of 236 amino acids and predicts a molecular mass of 25.5 KDa. It migrates as an approximately 61-66 KDa band in SDS-PAGE under reducing conditions.
蛋白NP号:AAH25395.1
蛋白氨基酸序列:Met1-Arg247
蛋白标签:C-cleavage
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验一、简介:1 细胞质膜资料 1895年,Overton从研究细胞透性得出"细胞膜由连续的脂类物质组成"。 1925年 Gorter&Grendel:用脂单分子膜技术测定细胞膜中脂分子的总面积,提出:"细胞膜是由双层脂分子组成"。 1935年 Danielli&Davson:从测定膜的表面张力得出细胞膜的"三明治结构模型",即蛋白质-脂-蛋白质。 1959年 Robertson:用电镜观察生物膜提出
蛋白分离纯化概述(An overview of protein isolation)
Isolating a protein may be compared to playing a game of golf. In golf, the player is faced with a series of problems, each unique and yet similar to problems previously encountered. In facing each problem the player must analyse
In order to carry out detailed studies of a protein (structure,enzyme activity, amino acid sequence, etc) it has to be very pure How is a single protein isolated from all the other proteins in the cell
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