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100 µg
蛋白名称:IFN-alpha 13蛋白, IFN-alpha 13 protein
蛋白构建:A DNA sequence encoding the cynomolgus IFNA13 (G7NFW4) (Met1-Glu190) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured in antiviral assay using WISH human amnion cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.5-3 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Cys 25
蛋白分子量:The recombinant cynomolgus IFNA13 is a disulfide-linked homodimer. The reduced monomer comprises 407 amino acids and has a calculated molecular mass of 46.4 KDa.The apparent molecular mass of the protein is approximately 47 KDa respectively in SDS-PAGE.
蛋白NP号:G7NFW4
蛋白氨基酸序列:Met1-Glu190
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验adrenergic receptor/Arg389Gly MDR1/C3435T il-2 il-4 il-10 TNF alpha IFN gamma hOGG1 bcr\abl human serum albumin VEGF165 GalNacT agouti-related protein, AGRP PAI-1 MDR1 MRP1 OPN U6 Promoter GFP MDR1 exon7~11 MDR1 exon12/14/18/20/22
Strep-tag®技术是亲和纯化重组蛋白的常用工具,它基于自然界中最强的非共价相互作用之一,即生物素与链霉亲和素的相互作用。该纯化系统的突出特点是纯化过程温和、目的蛋白纯度高、特异性强、兼容多种表达系统,对于有挑战性蛋白的纯化十分有帮助,因此近些年来脱颖而出。 本文将从以下方面介绍Strep-tag®技术: 标签介绍 纯化原理 标签-配体 纯化步骤 系统特点 标签介绍 目前有2种广泛使用的Strep标签: 它们分别是: Strep-tag®II 8 aa小标签(Trp-Ser
tumor necrosis factor–{alpha} (TNF-{alpha}), interferon-beta (IFN-beta), and IFN-{gamma} (1). The authors observed that of ~200 miRNAs examined, only miR-155 increased in amount in response to both TLR ligands and IFN-beta. miR-155 accumulated
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