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20 µg
蛋白名称:Kallikrein 7/KLK7蛋白, Kallikrein 7/KLK7 protein
蛋白构建:A DNA sequence encoding the mouse KLK7 (Q91VE3) (Met1-Arg249) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (AnaSpec, Cat#27114). The specific activity is >70 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 22
蛋白分子量:The recombinant mouse KLK7 comprises 239 amino acids and has a predicted molecular mass of 26.5 kDa. The apparent molecular mass of the protein is approximately 31 and 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:Q91VE3
蛋白氨基酸序列:Met1-Arg249
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验蛋白质技术中常常要用到lysis buffer,各个实验室的lysis buffer的配方是不同的,开设个专题,希望大家详细谈谈自己使用的lysis buffer的配方,以及各个组成成分的作用,方便广大蛋白质战友。 先介绍一下我们用的: PBS 缓冲液,不用多说; TRITON X-100 Triton X-100中文名为曲拉通X-100,分子式为t-Oct-C6H4-(OCH2CH2)xOH, x=9-10,平均分子量为647。 常用的非离子性去垢剂,主要
-like serine proteases bind to Benzamidine Sepharose 6B and can thus be used for purification and/ or removal of these substances. Trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein
-like serine proteases bind to Benzamidine Sepharose 6B and can thus be used for purification and/ or removal of these substances. Trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein
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