- ￥2310 - 3870
- Sino Biological
- 北京
- 80221-R08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Rat ALCAM / CD166 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
50.00 µg/100.00 µg
| 规格： | 50.00 µg | 产品价格： | ￥2310.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥3870.0 |
蛋白名称：CD166/ALCAM蛋白, CD166/ALCAM protein
蛋白构建：A DNA sequence encoding the rat ALCAM (O35112) (Met1-Lys527) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：Immobilized rat ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc (Cat:50711-M02H), The EC50 of mouse CD6-Fc (Cat:50711-M02H) is 0.08-0.18 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Trp 28
蛋白分子量：The recombinant rat ALCAM comprises 511 amino acids and predicts a molecular mass of 57.5 kDa. The apparent molecular mass of the recombinant protein is approximately 68-91 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：O35112
蛋白氨基酸序列：Met1-Lys527
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
). 4. In addition to studying the response of BMMs isolated from knock-out mice, theDCC can be used to characterize the chemotactic response of cells expressingtagged proteins introduced either by microinjection of recombinant protein ordeoxyribonucleic acid (16
in recombinant Escherichia coli, Biochem. J. 358 (2001) 263-268. 35. G. Lemercier, N. Bakalara, X. Santarelli, On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli, J
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