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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human ERBB2 Protein(Site-Specific PE Conjugation,ECD,His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20.00 µg/100.00 µg
| 规格: | 20.00 µg | 产品价格: | ¥1580.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥3480.0 |
蛋白名称:Interferon alpha 1/IFNA1蛋白, Interferon alpha 1/IFNA1 protein
蛋白构建:A DNA sequence encoding the rat IFNA1 (P05011) (Met1-Ser192) was expressed, fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 99 % as determined by SDS-PAGE
蛋白活性:Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 4-20 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Cys 24
蛋白分子量:The recombinant rat IFNA1 comprises 180 amino acids and predicts a molecular mass of 20.9 kDa. The apparent molecular mass of the rat IFNA1 is approximately 20-25 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:P05011
蛋白氨基酸序列:Met1-Ser192
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
of phosphorylation sites in proteins: Construction of a phosphorylatable human interferon alpha. Proc. Natl. Acad. Sci. U.S.A. 86:558‐562. Loizos, N. and Darst, S.A. 1998. Mapping protein
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
-19]; xChIP:用来研究DNA及低结合力蛋白,采用甲醛或紫外线进行 DNA 和蛋白交联,超声波片段化染色质,然后进行片段富集及后续分析,适用于多数非组蛋白的蛋白,例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后,对 DNA 进行 PCR 扩增,验证目标序列的存在。除验证实验外,ChIP DNA也可以进行测序分析,这种方法被称为 ChIP-seq [22] ;也可做芯片分析,这种方法被称为 ChIP on CHIP 或 ChIP
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