Fas / CD95 蛋白|Fas / CD95 protein|Fas / CD95(Cynomolgus, Fc Tag)

食蟹猴 Fas/CD95蛋白|Fas/CD95 protein|Fas/CD95(Cynomolgus, C-human IgG1-Fc)（产品说明）
蛋白名称：Fas/CD95蛋白, Fas/CD95 protein
蛋白构建：A DNA sequence encoding the cynomolgus FAS (F6V1W6) (Met1-Asp173) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-3 μg/mL in the presence of 200 ng/mL recombinant human Fas ligand. 2. Measured by its binding ability in a functional ELISA. Immobilized human His-FASLG (Cat：10244-H07Y) at 10 μg/ml (100 μl/well) can bind Cynomolgus FAS-Fc. The EC50 of Cynomolgus FAS-Fc is 0.06-0.14 μg/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gln 26
蛋白分子量：The recombinant cynomolgus FAS is a disulfide-linked homodimer. The reduced monomer comprises 389 amino acids and has a calculated molecular mass of 43.8 KDa.The apparent molecular mass of the protein is approximately 47-57 KDa in SDS-PAGE.
蛋白NP号：F6V1W6
蛋白氨基酸序列：Met1-Asp173
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.