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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Rat IFN-alpha / IFNA1 / IFN Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Rat IFN-alpha / IFNA1 / IFN Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the rat IFNA1 (P05011) (Met 1-Ser 192) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 90 % as determined by SDS-PAGE
蛋白活性:Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 1-10 μg/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Cys 24
蛋白分子量:The recombinant rat IFNA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 410 amino acids and predicts a molecular mass of 46.4 kDa. The apparent molecular mass of the rat IFNA1/Fc monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:P05011
蛋白氨基酸序列:Met1-Ser192
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验.7. Wang, X. et al. 2011. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5. Sci Rep1: 102 (DOI:10.1038/srep00102).8. Wang, X. and Zhang, S. 2011. Production of a bioengineered G-protein coupled receptor of human
1. 实验所需试剂 1.1 RAT IFN-GMA CBA FLEX SET (558305) 规格:100TST 组成:Rat IFN-γ 标准品(4ml稀释TOP浓度10000pg/ml),Rat IFN-γ PE 检测二抗试剂,Rat IFN-γ捕捉微球(定位:A6) 1.2 RAT IL-10 CBA FLEX SET (558306) 规格:100TST 组成:Rat IL-10 标准品(4ml稀释
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
, and analysis. A protocol is described for enhanced detection of low?affinity interactions by generating multivalent complexes using Fc?fusion bait proteins and protein A microbeads, along with a statistical method for hit scoring and identification
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